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The ortho-toluene sulfonamide is divided from the solvent of sulfonyl chloride, then oxidized to anthranilic acid, then heated and cyclized to be saccharin. Groups of 25 male and twenty five feminine Charles River CD mice, 8 months of age, received eating plans that contains sodium saccharin (containing 345 mg/kg (ppm) ortho-toluenesulfonamide) at